Article: Biodegradation of polyaromatic hydrocarbons by recombinant bacteria containing Alasan gene Journal: International Journal of Environment and Pollution (IJEP) 2009 Vol.38 No.4 pp.415 - 424 Abstract: Alasan, the bioemulsifier of Acinetobacter radioresistens KA53, is a high molecular-mass complex of an alanine-containing polysaccharide and three proteins. This gene was isolated from the A. radioresistens using PCR and the amplified product (850 bp) was cloned using PGEMT cloning kit. DNA sequence was carried out for the amplification and sequence analysis showed that the PCR product was highly similar to the OMPA precursor protein which called Alan. Subcloning was carried out into pTRAC expression vector and the purified protein was used in mineralisation of the polyaromatic hydrocarbon. As well as the recombinant E. coli cells were cultured directly on MSM containing four polyaromatic hydrocarbon using real time method (phenol, naphthalene, phenantherene and antherathene). The obtained results showed that no difference between the purified protein and the using of the recombinant cells directly with the presence of the inducer like IPTG. Both of the purified protein and the recombinant cells showed high degradation rates for (anthrathene and phenantherene). Inderscience Publishers - linking academia, business and industry through research

Title: Biodegradation of polyaromatic hydrocarbons by recombinant bacteria containing Alasan gene

Authors: M.M.M. Ahmed, Elsayed E. Hafez, M.A. El-Saadani

Addresses: Nucleic Acids Research Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), Mubarak City For Scientific Research and Technology Applications, Alexandria, Egypt. ' Department of Plant Molecular Pathology, Arid Lands Institute, Mubarak City For Scientific Research and Technology Applications, Alexandria, Egypt. ' Mubarak City for Scientific Research and Biotechnology Applications, Alexandria, Egypt

Abstract: Alasan, the bioemulsifier of Acinetobacter radioresistens KA53, is a high molecular-mass complex of an alanine-containing polysaccharide and three proteins. This gene was isolated from the A. radioresistens using PCR and the amplified product (850 bp) was cloned using PGEMT cloning kit. DNA sequence was carried out for the amplification and sequence analysis showed that the PCR product was highly similar to the OMPA precursor protein which called Alan. Subcloning was carried out into pTRAC expression vector and the purified protein was used in mineralisation of the polyaromatic hydrocarbon. As well as the recombinant E. coli cells were cultured directly on MSM containing four polyaromatic hydrocarbon using real time method (phenol, naphthalene, phenantherene and antherathene). The obtained results showed that no difference between the purified protein and the using of the recombinant cells directly with the presence of the inducer like IPTG. Both of the purified protein and the recombinant cells showed high degradation rates for (anthrathene and phenantherene).

Keywords: Alasan gene; recombinant bacteria; biodegradation; PCR; polyaromatic hydrocarbons; polysaccharides; proteins; E. coli cells.

DOI: 10.1504/IJEP.2009.027273

International Journal of Environment and Pollution, 2009 Vol.38 No.4, pp.415 - 424

Published online: 19 Jul 2009 *

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Title: Biodegradation of polyaromatic hydrocarbons by recombinant bacteria containing Alasan gene

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