Title: Ultra-sensitive protein detection using single molecule arrays (SiMoA): the potential for detecting single molecules of botulinum toxin

Authors: David C. Duffy

Addresses: Quanterix Corporation, 113 Hartwell Avenue, Lexington, MA 02421, USA

Abstract: Single molecule arrays (SiMoA) make it possible to detect subfemtomolar concentrations of proteins in complex samples. We describe the basis of SiMoA and its potential application in the detection of proteins, such as botulinum neurotoxin (BoNT), from pathogenic bacteria. We also provide a concept for an integrated handheld SiMoA device for the detection of very low concentrations of BoNT in the field.

Keywords: single molecule arrays; SiMoA; single molecules; proteins; digital ELISA; botulinum neurotoxins; BoNTs; microfluidics; botulism; protein detection; clostridium botulinum.

DOI: 10.1504/TBJ.2012.050200

The Botulinum Journal, 2012 Vol.2 No.2, pp.164 - 167

Published online: 30 Oct 2014 *