Construction of 2D and 3D biomolecules structures using fs lasers Online publication date: Fri, 25-Apr-2008
by Maria Farsari, Valentina Dinca, Maria Dinescu, Savas Georgiou, Costas Fotakis
International Journal of Nanomanufacturing (IJNM), Vol. 1, No. 6, 2007
Abstract: Bio-microarray fabrication has been the focus of much research in recent years, as they are envisaged to play an important part in genomic studies, protein identification, drug discovery and screening. We demonstrate both 2D and 3D biomolecules patterning using techniques which enable the construction of arbitrary two and three-dimensional shapes, not restricted to array-based shapes. For the 2D printing, Laser-Induced Forward Transfer (LIFT) is employed to deposit controlled and viable micropatterns of the Horseradish Peroxidase (HRP) and the functionality of the transferred materials is shown. For the 3D printing, firstly microstructures are made employing multiphoton polymerisation and then biotin is subsequently immobilised on the surface of the structures by excimer laser photo-activation of photobiotin and further exposed to fluorescently labelled streptavidin. The specificity of the binding is demonstrated.
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