The preparation and characteristic of poly(lactide co-glycolide) microspheres as novel antigen delivery systems
by Hsueh-Ju Lin; Ji-Hung Wang; Chun-Yu Wang; Yi-Chang Wu
International Journal of Nanotechnology (IJNT), Vol. 10, No. 10/11, 2013

Abstract: Polymeric microspheres with encapsulated antigens or medicine have become well established in the last decade as potent delivery systems. Our novel PLGA microspheres were prepared using a w/o/w solvent evaporation process in the presence of the anionic surfactants, including poly-L-lysine (PLL), cetyltrimethylammonium (CTAB), polyethyleneimine (PEI). High lamellae incorporation efficiency (97%), protein-adsorbed on PLA lamellae capacity (240 μg/mg), efficiency (93%) and encapsulated by PLGA formatted microspheres yield rate (77%) are successfully produced. The PEI modified PLA lamellae (240.2, 20) have more significantly increased proteins loading capacity (μg/mg) and in vitro 50% amount release time (day) than CTAB-modified (83.8, 0.5), PLL-modified (26.0, 0.5), and unmodified lamellae (10.1, 0.3), respectively. Also the sustained release effect over four weeks of FTIR-OVA microspheres was obtained by PEI modified PLA lamellae. In vitro release profiles show more active protein sustain release from PEI modified PLA lamellae than OVA-PLA only microspheres. Antigen binding to the charged PLGA microspheres was influenced by both electrostatic interaction and by other mechanisms, including hydrophobic interactions.

Online publication date: Sun, 08-Dec-2013

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