Detection of interleukin-8 mRNA using a gold nanowire-based biosensor with a stem-loop probe Online publication date: Sun, 08-Mar-2015
by Cao Huu Tien; Tran Van Man; Ha Van Linh; Pham Xuan Thanh Tung; Do Thi Thanh Thuy; Le Van Hieu
International Journal of Nanotechnology (IJNT), Vol. 12, No. 5/6/7, 2015
Abstract: We report a signal-off, electrochemical DNA (E-DNA) sensor for detection of salivary mRNA employing a stem-loop probe. One end of a stem-loop DNA is immobilised onto a gold electrode via a terminal thiol modification (forming a self assembled monolayer) with the other end bearing a redox-reporting methylene blue to create an E-DNA sensor. In the absence of target, the stem structure of the probe DNA holds the redox tag in proximity to the electrode, producing a large faradaic current. Hybridisation to a complementary oligonucleotide removes the redox tag from the electrode, significantly decreasing this current and supporting the ready detection of specific DNA sequences. The observed signal gain is sufficient to achieve a demonstrated detection limit of 200 pM, which is among the best reported for signal-off electrochemical DNA detection. This suggests that E-DNA signalling arises from binding-linked changes.
Existing subscribers:
Go to Inderscience Online Journals to access the Full Text of this article.
If you are not a subscriber and you just want to read the full contents of this article, buy online access here.Complimentary Subscribers, Editors or Members of the Editorial Board of the International Journal of Nanotechnology (IJNT):
Login with your Inderscience username and password:
Want to subscribe?
A subscription gives you complete access to all articles in the current issue, as well as to all articles in the previous three years (where applicable). See our Orders page to subscribe.
If you still need assistance, please email subs@inderscience.com
Our Blog 
Follow us on Twitter 
Visit us on Facebook