Bioconversion of Caulerpa racemosa derived galactose rich fraction to D-Tagatose using L-arabinose isomerase from Lactobacillus plantarum MTCC1407
by J. Jayamuthunagai; P. Gautam; M. Chakravarthy; G. Srisowmeya
International Journal of Materials and Product Technology (IJMPT), Vol. 55, No. 1/2/3, 2017

Abstract: A new source of galactose isolated from Caulerpa racemosa is used as raw material for D-tagatose conversion. D-galactose from Caulerpa derived galactose rich fraction (C-GF), which is 99.1% pure is employed in enzymatic bioconversion for D-tagatose using L-arabinose isomerase (l-AI) from Lactobacillus plantarum MTCC1407. The enzyme fraction with l-AI activity is purified to an apparent homogeneity evident on SDS-PAGE with a molecular mass of 59 kDa. Maximum l-AI activity of enzyme was observed at 50°C, pH 7.0 with 20 mM Mg²⁺ as cofactor. The potential of C-GF to act as D-tagatose feedstock was tested with varying concentrations of C-GF in 50 mM phosphate buffer added with obtained l-AI enzyme. This is compared with the results of pure D-galactose conversion using the same l-AI for better understanding of C-GF as tagatose raw material. Maximum equilibrium of 34.5% tagatose to C-GF galactose was obtained after 40 h producing 0.33 g tagatose/g galactose in C-GF.

Online publication date: Sun, 09-Jul-2017

The full text of this article is only available to individual subscribers or to users at subscribing institutions.

 
Existing subscribers:
Go to Inderscience Online Journals to access the Full Text of this article.

Pay per view:
If you are not a subscriber and you just want to read the full contents of this article, buy online access here.

Complimentary Subscribers, Editors or Members of the Editorial Board of the International Journal of Materials and Product Technology (IJMPT):
Login with your Inderscience username and password:

Username:        Password:          Forgotten your password?

Want to subscribe?
A subscription gives you complete access to all articles in the current issue, as well as to all articles in the previous three years (where applicable). See our Orders page to subscribe.

If you still need assistance, please email subs@inderscience.com