Title: Dynamic basis of mRNA processing in male germline stem cell pluripotency and reprogramming by alternative polyadenylation

Authors: Praveen Kumar Guttula; Mukesh Kumar Gupta

Addresses: Department of Biotechnology and Medical Engineering, National Institute of Technology (Rourkela), Rourkela, Sundargarh, Odisha, India ' Department of Biotechnology and Medical Engineering, National Institute of Technology (Rourkela), Rourkela, Sundargarh, Odisha, India

Abstract: Male Germline Stem (GS) cells are responsible for sperm production in males throughout the adulthood. Under in vitro culture conditions, these cells can undergo the reprogramming to generate Germline Pluripotent Stem (GPS) cells. However, the mechanism of reprogramming is elusive. The Alternative Polyadenylation (APA) pattern in GS and GPS cells was identified using APA Detect and 3' Untranslated Regions (3'-UTR) length was analysed in differentially expressed mRNAs. Results showed that, APA events regulated the expression of 78 genes and caused the shortening of 3'-UTR during the reprogramming. The APA regulated genes were found to be involved in the regulation of mRNA processing and splicing with shortened 3'-UTR. 28 miRNAs were found to target the APA-regulated genes. These results suggest that APA events might coordinate the reprogramming of GS cells to GPS cells by dynamic processing of 3'-UTR length in differentially expressed mRNAs through miRNAs.

Keywords: alternate polyadenylation; germline stem cells; germline pluripotent stem cells; reprogramming; microRNA; polyadenylation site.

DOI: 10.1504/IJDMB.2021.122850

International Journal of Data Mining and Bioinformatics, 2021 Vol.25 No.3/4, pp.145 - 160

Received: 13 Dec 2020
Accepted: 01 Nov 2021
Published online: 13 May 2022 *

Full-text access for editors Full-text access for subscribers Purchase this article Comment on this article