Article: Development, characterisation and cytotoxicity studies of erlotinib entrapped gellan gum nanoparticles Journal: International Journal of Nanoparticles (IJNP) 2022 Vol.14 No.1 pp.47 - 68 Abstract: Gellan gum is an anionic, water-soluble polysaccharide produced by the bacterium Sphingomonas paucimobilis ATCC 31461 via the fermentation process. The gellan gum-erlotinib (GgE) nanoparticles were synthesised by the emulsion cross-linking method. The synthesised GgE nanoparticles were characterised by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), differential scanning calorimetry (DSC), thermogravimetric (TGA), particle size and zeta potential analysis. The influence of gellan, drug and calcium chloride concentration on drug loading and entrapment efficiency was evaluated. In-vitro release behaviour' of erlotinib was examined by dialysis bag technique. Further, to assess the potential anticancer efficacy of GgE nanoparticles, in-vitro cytotoxicity studies on HepG2 cell lines by SRB assay were carried out. SEM results showed spherical smooth-surfaced nanoparticles. FTIR confirmed the interaction between polymer matrix and erlotinib. The drug loading and entrapment efficiency of GgE nanoparticles increased with gellan and drug concentration. The GgE nanoparticles showed pH-responsive drug release leading to faster release of erlotinib at acidic buffer than neutral and alkaline. The GgE nanoparticles exhibited a cytotoxic effect on HepG2 cells and their cytotoxicity was more significant than free erlotinib and gellan gum. Inderscience Publishers - linking academia, business and industry through research

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Title: Development, characterisation and cytotoxicity studies of erlotinib entrapped gellan gum nanoparticles

Authors: Soumiya Sukumar; Santhiagu Arockiasamy; Manjusha Chemmattu Moothona

Addresses: School of Biotechnology, National Institute of Technology Calicut, Calicut, Kerala 673601, India ' School of Biotechnology, National Institute of Technology Calicut, Calicut, Kerala 673601, India ' School of Biotechnology, National Institute of Technology Calicut, Calicut, Kerala 673601, India

Abstract: Gellan gum is an anionic, water-soluble polysaccharide produced by the bacterium Sphingomonas paucimobilis ATCC 31461 via the fermentation process. The gellan gum-erlotinib (GgE) nanoparticles were synthesised by the emulsion cross-linking method. The synthesised GgE nanoparticles were characterised by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), differential scanning calorimetry (DSC), thermogravimetric (TGA), particle size and zeta potential analysis. The influence of gellan, drug and calcium chloride concentration on drug loading and entrapment efficiency was evaluated. In-vitro release behaviour' of erlotinib was examined by dialysis bag technique. Further, to assess the potential anticancer efficacy of GgE nanoparticles, in-vitro cytotoxicity studies on HepG2 cell lines by SRB assay were carried out. SEM results showed spherical smooth-surfaced nanoparticles. FTIR confirmed the interaction between polymer matrix and erlotinib. The drug loading and entrapment efficiency of GgE nanoparticles increased with gellan and drug concentration. The GgE nanoparticles showed pH-responsive drug release leading to faster release of erlotinib at acidic buffer than neutral and alkaline. The GgE nanoparticles exhibited a cytotoxic effect on HepG2 cells and their cytotoxicity was more significant than free erlotinib and gellan gum.

Keywords: gellan gum; erlotinib; sphingomonas paucimobilis; GgE nanoparticles; in-vitro drug release; cytotoxicity.

DOI: 10.1504/IJNP.2022.122934

International Journal of Nanoparticles, 2022 Vol.14 No.1, pp.47 - 68

Accepted: 21 Nov 2021
Published online: 17 May 2022 *

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Title: Development, characterisation and cytotoxicity studies of erlotinib entrapped gellan gum nanoparticles

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