Title: Transcriptome analysis of muskmelon respond to cadmium stress

Authors: Yunqiang Wang; Zhen Lei; Xiaoqian Liu; Zhengkang Zou; Licong Yi; Yu Gong; Zhaoyi Dai; Junli Li

Addresses: Institute of Economic Crops, Hubei Academy of Agricultural Science, Wuhan 430064, China ' School of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of Technology, Wuhan 430070, China ' School of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of Technology, Wuhan 430070, China ' School of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of Technology, Wuhan 430070, China ' Institute of Economic Crops, Hubei Academy of Agricultural Science, Wuhan 430064, China ' Institute of Economic Crops, Hubei Academy of Agricultural Science, Wuhan 430064, China ' Institute of Economic Crops, Hubei Academy of Agricultural Science, Wuhan 430064, China ' School of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of Technology, Wuhan 430070, China

Abstract: Cadmium (Cd) contamination is the most serious and largest heavy metal pollution of China. It is necessary to explore the mechanism of Cd poisoning and the mechanism of resistance to Cd stress in crops. In the study, Illumina sequencing technology was used for transcriptome high-throughput sequencing. The molecular mechanism of Cd stress in muskmelon was investigated using gene ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG) enrichment analysis. In addition, through the detection of physiological indexes of muskmelon leaves, it was found that chlorophyll content and POD activity were considerably reduced. In addition, the related genes were significantly up-regulated exposed to Cd. In the study, the main pathways and genes of muskmelon response to Cd stress were analysed, providing a theoretical foundation for future research into the mechanism of Cd stress response.

Keywords: muskmelon; RNA-seq; transcriptome; high-throughput sequencing; Cd stress; differentially expressed genes; GO; gene ontology; KEGG; Kyoto encyclopedia of genes and genomes; physiological indexes; pathways.

DOI: 10.1504/IJEP.2021.130307

International Journal of Environment and Pollution, 2021 Vol.70 No.1/2, pp.33 - 59

Received: 01 Aug 2021
Received in revised form: 10 Aug 2022
Accepted: 05 Sep 2022
Published online: 17 Apr 2023 *

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