Title: Novel Poly (3-hydroxybutyrate) production using Bacillus subtilis NG220 and watermelon derived substrates

Authors: Sudad A. Dayl; Rawa G. Yousuf

Addresses: Department of Chemistry, College of Science, University of Baghdad, Iraq ' Biochemical Engineering Department, Al-Khawarizmi College of Engineering, University of Baghdad, Iraq

Abstract: Poly (3-hydroxybutyrate) (PHB) is a typical microbial bio-polyester reserve material; known as “green plastics”, which produced under controlled conditions as intracellular products of the secondary metabolism of diverse gram-negative/positive bacteria and various extremophiles archaea. Although PHB has properties allowing being very attractive, it is too expensive to compete with conventional and non-biodegradable plastics. Feasibility of this research to evaluate the suitability of using a watermelon-derived media as an alternative substrate for PHB synthesis under stress conditions was examined. Results, include the most nutrients extraction, indicated that the watermelon seeds contain a high content of nutrients makes them a promising candidate of substrate material for microbial cell growth. As well as a considerable quantity of PHB granules can be accumulated without adding any nutrients subjunctive. The total carbon concentration was 11.8 g/l, giving the highest PHB content of 65% and PHB concentration 4.5 g/l and total biomass 6.65 g/l.

Keywords: Poly (3-hydroxybutyrate); PHB; watermelon seeds; Bacillus subtilis NG220; agricultural waste.

DOI: 10.1504/IJEWM.2023.134508

International Journal of Environment and Waste Management, 2023 Vol.32 No.4, pp.455 - 468

Received: 01 Jun 2020
Accepted: 24 May 2021
Published online: 26 Oct 2023 *

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