Title: Investigation of photocatalytic effects and extraction of genomic DNA from Staphylococcus aureus through Fe₃O₄/SiO₂/TiO₂ magnetic nanoparticles

Authors: Farzaneh Firoozeh; Mohammadreza Rezaei Yazdi; Mohammad Zibaei; Hadiseh Rostami; Ali Sobhani Nasab; Azad Khaledi; Farzad Badmasti

Addresses: Department of Microbiology, School of Medicine, Alborz University of Medical Sciences, Karaj, Iran; Evidence-Based Phytotherapy and Complementary Medicine Research Centre, Alborz University of Medical Sciences, Karaj, Iran ' Department of Microbiology, Faculty of Life Sciences, North Tehran Unit, Islamic Azad University, Tehran, Iran ' Evidence-Based Phytotherapy and Complementary Medicine Research Centre, Alborz University of Medical Sciences, Karaj, Iran; Department of Parasitology and Mycology, Faculty of Medicine, Alborz University of Medical Sciences, Karaj, Iran ' Faculty of Basic Sciences, Islamic Azad University, Science and Research Branch, Tehran, Iran ' Physiology Research Centre, Institute for Basic Sciences, Kashan University of Medical Sciences, Kashan, Iran ' Department of Microbiology and Immunology, School of Medicine, Kashan University of Medical Sciences, Kashan, Iran ' Department of Bacteriology, Pasteur Institute of Iran, Tehran, Iran

Abstract: Staphylococcus aureus has been considered as one of the main pathogens that cause various diseases. Therefore, access to fast and reliable DNA-based methods is crucial for the detection and identification of this bacterium. DNA extraction and purification are fundamental primary steps in almost all molecular biology studies. Therefore, the purpose of this work is utilising Fe₃O₄/SiO₂/TiO₂ magnetic nanoparticles to extract genomic DNA of Staphylococcus aureus. This paper contains extracting genomic DNA from standard strain of Staphylococcus aureus ATCC 25923 using Fe₃O₄/SiO₂/TiO₂ nanostructures. The quality of extracted DNA was evaluated after electrophoresis on gel agarose, also DNA purity and concentrations were measured by a NanoDrop spectrophotometer. The concentration of genomic DNA extracted by Fe₃O₄/SiO₂ magnetic nanoparticles from Staphylococcus aureus strain ATCC 25923 was 131.635 ng/μL. Also, A260/280 and A260/230 values of mentioned DNA were ranged 1.7 to 1.8 and 2 to 2.2 respectively. The obtained results showed that the DNA extracted by the synthesised magnetic nanoparticles has an acceptable concentration and purity for subsequent molecular biology studies in this bacterium.

Keywords: Fe₃O₄/SiO₂/TiO₂; DNA extraction; Staphylococcus aureus; genomic DNA magnetic nanoparticles.

DOI: 10.1504/IJBET.2024.136372

International Journal of Biomedical Engineering and Technology, 2024 Vol.44 No.1, pp.64 - 79

Received: 12 Nov 2022
Accepted: 10 Feb 2023
Published online: 31 Jan 2024 *

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