Title: Construction of 2D and 3D biomolecules structures using fs lasers

Authors: Maria Farsari, Valentina Dinca, Maria Dinescu, Savas Georgiou, Costas Fotakis

Addresses: Institute of Electronic Structure and Laser, Foundation for Research and Technology, Vassilica Vouton, Hellas 71110, Heraklion, Greece. ' Institute of Electronic Structure and Laser, Foundation for Research and Technology, Vassilica Vouton, Hellas 71110, Heraklion, Greece. ' National Institute for Laser, Plasma and Radiation Physics, Bucharest, Romania. ' Institute of Electronic Structure and Laser, Foundation for Research and Technology, Vassilica Vouton, Hellas 71110, Heraklion, Greece. ' Institute of Electronic Structure and Laser, Foundation for Research and Technology, Vassilica Vouton, Hellas 71110, Heraklion, Greece.

Abstract: Bio-microarray fabrication has been the focus of much research in recent years, as they are envisaged to play an important part in genomic studies, protein identification, drug discovery and screening. We demonstrate both 2D and 3D biomolecules patterning using techniques which enable the construction of arbitrary two and three-dimensional shapes, not restricted to array-based shapes. For the 2D printing, Laser-Induced Forward Transfer (LIFT) is employed to deposit controlled and viable micropatterns of the Horseradish Peroxidase (HRP) and the functionality of the transferred materials is shown. For the 3D printing, firstly microstructures are made employing multiphoton polymerisation and then biotin is subsequently immobilised on the surface of the structures by excimer laser photo-activation of photobiotin and further exposed to fluorescently labelled streptavidin. The specificity of the binding is demonstrated.

Keywords: nonlinear photopolymerisation; laser induced forward transfer; LIFT; femtosecond lasers; direct writing; biotin; avidin; biomolecules patterning; microarrays; biomicroarrays; micropatterns; microstructures; biomolecule structures.

DOI: 10.1504/IJNM.2007.017994

International Journal of Nanomanufacturing, 2007 Vol.1 No.6, pp.762 - 770

Published online: 25 Apr 2008 *

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