Title: Bolaamphiphiles based novel liposomes: a possible approach for drug delivery

Authors: Bhuvaneshwar Vaidya, Amit K. Goyal, Kapil Khatri, Neeraj Mishra, Rishi Paliwal, Shivani Rai, Shailja Tiwari, Suresh P. Vyas

Addresses: Drug Delivery Research Laboratory, Department of Pharmaceutical Sciences, Dr. H. S. Gour Viswavidyalaya, Sagar-470003 M.P., India. ' Drug Delivery Research Laboratory, Department of Pharmaceutical Sciences, Dr. H. S. Gour Viswavidyalaya, Sagar-470003 M.P., India. ' Drug Delivery Research Laboratory, Department of Pharmaceutical Sciences, Dr. H. S. Gour Viswavidyalaya, Sagar-470003 M.P., India. ' Drug Delivery Research Laboratory, Department of Pharmaceutical Sciences, Dr. H. S. Gour Viswavidyalaya, Sagar-470003 M.P., India. ' Drug Delivery Research Laboratory, Department of Pharmaceutical Sciences, Dr. H. S. Gour Viswavidyalaya, Sagar-470003 M.P., India. ' Drug Delivery Research Laboratory, Department of Pharmaceutical Sciences, Dr. H. S. Gour Viswavidyalaya, Sagar-470003 M.P., India. ' Drug Delivery Research Laboratory, Department of Pharmaceutical Sciences, Dr. H. S. Gour Viswavidyalaya, Sagar-470003 M.P., India. ' Drug Delivery Research Laboratory, Department of Pharmaceutical Sciences, Dr. H. S. Gour Viswavidyalaya, Sagar-470003 M.P., India; Indo-Soviet Friendship College of Pharmacy, Moga (Punjab), 142001, India

Abstract: The present study was designed to develop a novel liposomal system containing dequalinium chloride (DEQ) as a charge-imparting constituent. Liposomes were prepared by cast film method with EPC:chol:DEQ molar ratio of 7:1:2. The novel liposomes were characterised for surface morphology, size and size distribution, entrapment efficiency and in vitro release study. Effect of osmotic shock was performed for establishment of liposomal bilayer stability and results demonstrated that incorporation of dequalinium chloride to the liposomal bilayer hindered the movement of entrapped solute. The hemolysis study was performed for estimation of its toxicological behaviour, if any. Cell-uptake studies showed that developed carrier could be useful for higher penetration in the cells compared to conventional liposomes. This being the first finding of its kind could open up a new era for the use of bolaamphiphiles like DEQ as an integral part of well established carrier like liposomes to provide them additional stability and efficacy.

Keywords: liposomes; dequalinium chloride; DEQ; bolaamphiphiles; drug delivery; surface morphology; size distribution; entrapment efficiency; in vitro release; osmotic shock; liposomal bilayer stability.

DOI: 10.1504/IJBNN.2010.034648

International Journal of Biomedical Nanoscience and Nanotechnology, 2010 Vol.1 No.2/3/4, pp.95 - 108

Published online: 13 Aug 2010 *

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