Title: Decolourisation of Turquoise HGN by anaerobic bacterial isolates from rumen enrichments

Authors: C.G. Morlett Chavez; N. Balagurusamy

Addresses: Facultad de Ciencias Quimicas, Universidad Autonoma de Coahuila Saltillo, Blvd. V. Carranza y J. Cardenas V., Saltillo, Coahuila, C.P. 25280 Mexico. ' Escuela de Ciencias Biologicas, Universidad Autonoma de Coahuila, Ciudad Universitaria, Carretera Torreón-Matamoros Km. 7.5, Torreon, Coahuila State, Mexico. C.P.27104

Abstract: Anaerobic bacteria capable of decolourising Turquoise HGN dye were isolated by roll tube technique from enrichments inoculated with rumen fluid and were tested for their decolourisation efficiency under different conditions. The presence of glucose in the culture medium favoured the decolourisation and specific dye reduction activity (equivalent to the reduction of 1 mg dye. hr^?1.mg^?1 of protein), but both were inhibited in the presence of yeast extract. The maximum growth and dye reduction activity was recorded at pH 7.0 and 30°C, and were in the range of 36.4–97.4 U and 68–82 U, respectively with glucose as co-metabolite.

Keywords: anaerobic bacteria; dye decolourisation; specific dye reduction; rumen fluid; turquoise HGN; yeast extract; synthetic dyes; wastewater treatment.

DOI: 10.1504/IJEWM.2012.044169

International Journal of Environment and Waste Management, 2012 Vol.9 No.1/2, pp.191 - 199

Published online: 13 Dec 2014 *

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