Title: Technical note: application of RAPD markers in fish. Part II: among and within families; Cichlidae (Freshwater), Mugilidae (Catadromous), Sparidae and Serranidae (Marine)
Authors: Bahy Ahmed Ali, Mohamed M.M. Ahmed, Samy Y. El-Zaeem
Addresses: Nucleic Acid Research Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), Mubarak City for Scientific Research & Technology Applications, Alexandria, Egypt. ' Nucleic Acid Research Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), Mubarak City for Scientific Research & Technology Applications, Alexandria, Egypt. ' Animal and Fish Production Department, Faculty of Agriculture, Saba-Bacha, Alexandria University, Alexandria, Egypt
Abstract: Random amplified polymorphic DNA (RAPD) analysis was applied to the four fish families in Egypt: Cichlidae (freshwater), Mugilidae (Catadromous), Sparidae (marine). Twenty random primers were used to assay polymorphisms within and among these families. Different RAPD fragment patterns were observed. Results demonstrated that there are great differences among the first three families. Also, the genetic difference within each family can be observed. Evidence is presented about RAPD marker useful for systematic investigation among and within different families, which live under different degrees of water salinities.
Keywords: fish families; freshwater; Catadromous; marine; RAPD; water salinity; random amplified polymorphic DNA; Egypt.
International Journal of Biotechnology, 2004 Vol.6 No.4, pp.393 - 401
Published online: 12 Oct 2004 *
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