Simulate the agarose gel of the receptor binding domain in Omicron's spike protein inserted into the pGEM®-T Easy vector using SpeI and SacI restriction enzymes Online publication date: Tue, 31-Oct-2023
by Ali Adel Dawood
International Journal of Computational Biology and Drug Design (IJCBDD), Vol. 15, No. 6, 2023
Abstract: The Omicron variant of SARS-CoV-2 spreads swiftly among humans because of the mutations it carries. Aim: The goal of this study was to find a full design of the polymerase chain reaction (PCR) protocol for determining SARS-CoV-2 using In silico tools. The receptor-binding domain (RBD) of the spike protein is a crucial component. Materials and Methods: RBD interacts with ACE2 receptors on the surface of respiratory lining cells. The linear RBD was inserted into the supercoiled pGEM®-T Easy vector using SpeI and SacI restriction enzymes. The RBD was amplified to perform the conventional PCR technique after designing specific primers. Results: The (pGEM®-T Easy-RBD Mut.) clone was created by inserting the Omicron RBD gene into a supercoiled pGEM®-T Easy commercial vector. The results were predicted using gel electrophoresis. Even though the classic PCR method takes time due to the electrophoresis procedure, it is a very efficient tool for detecting novel strains.
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